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Horses tested by competitive ELISA for on index ranch of equine piroplasmosis outbreak in southern Texas, USA, 2009
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*Division A contained all younger stock. Infection rates among younger animals were lower. The other 3 divisions contained mostly horses used for working cattle.

Ticks collected from horses on the index ranch were shipped alive to NVSL. Identifications were made by using morphologic characteristics, geographic distribution, biologic characteristics, and host associations ( 5 Discount Cheap Price New And Fashion Jean Atelier Laurent Jeans Clearance 2018 New Free Shipping In China qG2sSYQ
). NVSL received ticks from 228 horses; >1 species was present on 41 animals. The dominant tick, Amblyomma cajennense , was collected from 180 (78.9%) horses ( Table 2 ).

Tick species found on horses at index ranch of equine piroplasmosis outbreak in southern Texas, USA, 2009
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*Of 228 horses sampled, 41 had >1 species of tick present.

All ticks were identified and sent to the Agricultural Research Service, Animal Disease Research Unit, USDA (Pullman, WA, USA) for transmission studies. Live males and partially fed females were pooled by species and held at 25°C and a relative humidity of 98% until they were allowed to reattach and feed on uninfected horses.

A total of 104 A. cajennense ticks (45 male and 79 female) were placed on a horse on October 30, 31, and November 2, 2009. These ticks had been removed from 73 horses on the index ranch, of which 68 (93.2%) were seropositive for T. equi . Females were allowed to reattach and feed until repletion; males were removed when all females were replete. All ticks were removed by November 18, 2009. Twenty-four fully engorged females and 3 live males were recovered. The horse had a fever (>39°C) 14 days after the ticks were first applied. Parasitized erythrocytes on a stained blood smear peaked at 0.3% on day 17. No other clinical signs of infection were evident. Serologic analysis and PCR ( 10 ) confirmed T. equi infection.

Twenty-nine D. variabilis ticks (12 male and 17 female) were placed on a second uninfected horse on October 30 and 31 and November 2 and 12, 2009. These ticks had been removed from 17 horses, of which 11 were seropositive and 1 was seronegative for T. equi ; 5 had an unknown infection status. All ticks were removed by November 24, 2009. Six fully engorged females and 7 live males were recovered. This horse had a slight fever (39°C) 15 days after tick attachment but otherwise showed no clinical signs. No organisms were found in blood smears, but this horse was positive for T. equi by PCR 42 days after the first ticks were attached and by competitive ELISA 87 days after tick attachment.

Walking separates these two states of the creative process. It allows time to expand on what you’ve already worked on, and removes youfromwork so you canthink clearly.

For the layman, this creates a convincing argument that trying to force the entire process to occurat your desk is a mistake—separating work from consumption and incubation becomes far easier when the locations are different.

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and do your best thinking somewhere else.

Don’t End Your Day Empty

Before I began a deeper investigation into creative thinking, it was my personal habit to always try to end the day “empty.” I would actively try to exhaust any project where I was making progress.

To my surprise, some writers like Ernest Hemingway ardentlydisagree with this, offering contrarian advice to instead never end your dayempty:

"I had learned already never to empty the well of my writing, but always to stop when there was still something there in the deep part of the well, and let it refill at night from the springs that fed it."

Stopping while you still have something to sayallows you to start the next day knowing exactly where to begin.

Interesting how this all ties into Hemingway’s closing ritual . With the next morning’s task already defined, Hemingwaycould domeaningful work as soon as he rose. As an avid walker himself, this also made his laterafternoon walks all the more effective.

closing ritual

Many studies would argue that unstructured “daydreaming” isn’t as important to creativity as you might think; unconscious thought can only really be of any help whenithas lots of information to incubate (in other words, only if you’ve worked on the problem extensively).

If you recall Beethoven’s and others schedules, most did not begin their day with incubation , most preferred tostart with thework, just like Hemingway.

Perhaps notending your day completely empty can relieve you of “what do I work on?” stress inthe morning. You’ll also be able to begin the day doing the work needed to get to inspiration.

Stop before you are empty, define your goals the night before, and step away from problems midday with a contemplative walk. It might not turn you into a famous writer, but see if it doesn’t help with your creative process.

Getting Your Ideas Out to the World

One additional lesson I walked away with throughexploring this topic wasthereminderthat creativity is nothing without creation.

Ideas, in this sense, are more like wishes. They would ideally result in creative output, but unless you can execute they will forever remain something that “could have been,” now relegated to the myriad of other thingsstuck in your head.

What use is inspiration if it never manifests into art? What’s the point of a thoughtful strategy if it’s never applied? What good are scientific insights if they aren’t fleshed out and published?

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